Figure 1 from Targeting Pancreatic Cancer Cell Stemness by Blocking Fibronectin-Binding Integrins on Cancer-Associated Fibroblasts
Year: 2025
Type: other
Abstract: Tumor cells utilize CAF-produced FN to overcome isolation stress. A, CAFs enhance tumor initiation via FN (orthotopic pancreas cancer model). Luciferase-expressing KP4 PDAC cells were orthotopically injected (with or without CAF-1299) into the pancreas of nu/nu mice. After 3 weeks, luciferase imaging was performed to survey tumor establishment in the pancreas. B, FN knockdown prevents CAF assembly of COL fibers. CAF-1299 and CAF-1424 cells were treated with siRNA for a scramble control vs. FN. After 72 hours, IF staining shows FN and COL content. Images are representative of at least three independent experiments. C, Knockdown of FN in CAFs increases soluble COL in media. CAF-1299 cells were cultured for 72 hours, and then the conditioned media and cell lysates were collected and processed for immunoblotting to confirm FN knockdown and assess the level of soluble COL secreted by the cells into the media. si-CTRL, siRNA Universal Negative Control; si-FN, siRNA-mediated knockdown of FN1.
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4407037722