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Figure 2 from Targeting Pancreatic Cancer Cell Stemness by Blocking Fibronectin-Binding Integrins on Cancer-Associated Fibroblasts

Year: 2025

Type: other

Abstract: FN-binding integrins mediate ECM production. Knockdown of FN or FN-binding integrins in CAFs prevents the formation of FN and COL fibers. CAF-1299 cells were treated with siRNA for a scramble control vs. FN, ITGA5, or ITGB3. After 72 hours, IF staining shows FN and COL content. The top set of images were not permeabilized. Images are representative of at least three independent experiments. Blots confirm knockdown. si-CTRL, CAFs with siRNA for a Universal Negative Control; si-FN, CAFs with siRNA-mediated knockdown of FN1; si-α5, CAFs with ITGA5 knockdown; si-β3, CAFs with ITGB3 knockdown.

DOI: 10.1158/2767-9764.28322543

Authors: Chengsheng Wu, Tami Von Schalscha, Diva Sansanwal, Chen Qian, Qinlin Jiang, Ryan M. Shepard, Hiromi I. Wettersten, Stephen J. McCormack, Sara M. Weis, David A. Cheresh

Language: English

Topic: Pancreatic and Hepatic Oncology Research

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4407037737

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