Figure 5 from Targeting Pancreatic Cancer Cell Stemness by Blocking Fibronectin-Binding Integrins on Cancer-Associated Fibroblasts
Year: 2025
Type: other
Abstract: Disrupting FN-binding integrins prevents the ability of CAFs to enhance tumor initiation. A, CAFs enhance tumor initiation via FN and FN-binding integrins (subcutaneous xenograft model). PANC1 human PDAC cells were injected subcutaneously (with or without CAF-1299) into the flank areas of nu/nu mice. Mice were monitored twice weekly to detect the earliest emergence of palpable tumors. The graph shows tumor take rate vs. time for 10–12 mice per group, using a volume of 100 mm3 (computed as length × width2) as the threshold for tumor take. At the endpoint of the experiment (day 56), tumors were harvested and prepared for histologic analysis. B, BsAb treatment reduces tumor stroma and increases necrosis. Tumor sections were stained using hematoxylin and eosin. Areas of stroma (S) and necrosis (N) are noted. C, BsAb treatment reduces tumor stiffness. Cryosections of tumors were analyzed using atomic force microscopy to evaluate tissue stiffness. Dots depict the mean value for each 20 × 20 μm region of interest (ROI). Fifteen ROIs were evaluated per tumor, for two tumors per group.
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4407037915