Work

A transcription factor-based biosensor for identification of the polyethylene terephthalate-degrading enzyme

Year: 2025

Type: article

Abstract: Screening and mining efficient polyethylene terephthalate (PET)-degrading enzyme is a promising strategy for plastic waste treatment and recycling. This invention provides a novel transcription factor-based biosensor method for identifying potential PET-degrading enzymes from the microorganisms. When PET is degraded by a specific enzyme to produce terephthalic acid (TPA), the transcription factor TphR recognizes the TPA substrate and then activates the transcription of downstream green fluorescent protein (GFP) reporter, generating measurable fluorescence signals in bacterial cells.

Source: Invention Disclosure

DOI: 10.1016/j.inv.2025.100038

Author: Shengwei Sun

Institution: KTH Royal Institute of Technology

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Responsible consumption and production

Open Access status: gold

Estimated APC paid: $820