Work

Novel fixed-target serial crystallography flip-holder for macromolecular crystallography beamlines at synchrotron radiation sources

Year: 2025

Type: article

Abstract: Synchrotron serial crystallography (SSX) is an emerging method for determining crystal structure at room temperature using synchrotron radiation facilities. Despite the various approaches available, reducing sample consumption, removing mother liquid from crystal solution, soaking small molecules for protein-ligand complex structure and prevention of sample dehydration are still challenging problems to be overcome in SSX. Therefore, we have developed a new flip-type fixed-target SSX sample holder for conventional protein crystallography beamlines based on nylon mesh and kapton film. The potential of the flip-holder was evaluated in an SSX experiment with lysozyme crystals at room temperature. About 19600 diffraction images were collected during 40 minutes using a repetition rate of 10 Hz at the 11C beamline of the Pohang Accelerator Laboratory, and the crystal structure of lysozyme was determined at 1.89 Å resolution. This straightforward flip-holder can be used in synchrotron beamlines for routine crystallography.

Source: Journal of Synchrotron Radiation

DOI: 10.1107/s1600577524011664

Authors: Do-Heon Gu, Dong Tak Jeong, Cheolsoo Eo, Pil-Won Seo, Jeong-Sun Kim, Suk-Youl Park

Institutions: Chonnam National University, Pohang University of Science and Technology, Pohang Accelerator Laboratory

Language: English

Topic: Enzyme Structure and Function

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $3,180

Funders: Australian Synchrotron, National Research Foundation, National Research Foundation of Korea, Ministry of Science and ICT, South Korea