Preparation of SARS-CoV-2 Saliva Samples Using an ARTIC V4.1 Tiled Amplicon Approach for Sequencing on Illumina Platforms v1
Year: 2024
Type: preprint
Abstract: This protocol describes how to prepare purified SARS-CoV-2 RNA from positive saliva samples for short-read sequencing. The initial targeted amplification utilizes the commercially available IDT ARTIC V4.1 tiled amplicon panel, followed by amplification with IDT custom-ordered fusion primers that follow the same amplicon scheme, allowing for further amplification of target molecules and eliminating the need for end repair and adapter ligation. Completion of this protocol should result in a clean, multiplexed DNA library pool ready for sequencing.
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4407126303