CellMAP: an open-source software tool to batch-process cell topography and stiffness maps collected with an atomic force microscope
Year: 2025
Type: article
Abstract: Atomic force microscopy (AFM) is the gold-standard technique to simultaneously map the morphology and viscoelastic properties of living cells. Although existing software tools, both open-source and from AFM manufacturers, can analyze cells individually, there is a growing need for fast and accessible codes to compile data from multiple cells into a single dataset. To address this, we present CellMAP, a user-friendly software tool that streamlines the batch-processing of AFM-derived topography and stiffness maps of living cells. Our analysis pipeline includes but is not limited to: flattening of the underlying substrate surface, filtering of outlier values, measurement of the cell surface and volume, and measurement of height and stiffness distributions. CellMAP can also generate a composite cell that reflects the height and stiffness properties of an entire cell population.
Source: BMC Bioinformatics
Authors: Antoine Allard, Maxime Liboz, Raphaël Crépin, Sid Labdi, Olek Maciejak, Michel Malo, Clément Campillo, Guillaume Lamour
Institutions: Centre National de la Recherche Scientifique, Université de Bordeaux, Université Paris-Saclay, Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement, CY Cergy Paris Université, Laboratoire Ondes et Matière d'Aquitaine, Université d'Évry Val-d'Essonne, Institut Universitaire de France
Language: English
Subfield: Atomic and Molecular Physics, and Optics
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,990
OpenAlex ID: W4407136152