Work

Engineering a genomically recoded organism with one stop codon

Year: 2025

Type: article

Abstract: to mitigate native UGA recognition, translationally isolating four codons for non-degenerate functions. Ochre thus utilizes UAA as the sole stop codon, with UGG encoding tryptophan and UAG and UGA reassigned for multi-site incorporation of two distinct non-standard amino acids into single proteins with more than 99% accuracy. Ochre fully compresses degenerate stop codons into a single codon and represents an important step toward a 64-codon non-degenerate code that will enable precise production of multi-functional synthetic proteins with unnatural encoded chemistries and broad utility in biotechnology and biotherapeutics.

Source: Nature

DOI: 10.1038/s41586-024-08501-x

Authors: Michael W. Grome, Michael T. A. Nguyen, Daniel W. Moonan, Kyle Mohler, Kebron Gurara, Shenqi Wang, Colin Hemez, Benjamin J. Stenton, Yunteng Cao, Felix Radford, Maya Kornaj, Jaymin Patel, Maisha Prome, Svetlana Rogulina, David Sozanski, Jesse Tordoff, Jesse Rinehart, Farren J. Isaacs

Institution: Yale University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $12,690

Funders: National Science Foundation, National Institutes of Health, Defense Advanced Research Projects Agency, National Institute of General Medical Sciences, National Institute of Biomedical Imaging and Bioengineering

Awards: Deciphering human signaling networks through synthetic activation of proteins in genomically recoded organisms with multiple open codons, G4208297642, Collaborative Research: Booting up a Mirror Cell, Programmable Keratinous Bio-adhesives for Recalcitrant Wound Recovery, G5931632516, G6666280201, G7152168685, Predoctoral Training Program in Genetics

OpenAlex ID: W4407157759

FWCI: 11.09

Cites: 70

Cited by: 44

Related to: 10