Salinity induced changes in esterase, peroxidase and alcohol dehydrogenase isozymes and leaf soluble proteins in salinity susceptible and salinity tolerant sugarcane genotypes
Year: 2025
Type: article
Abstract: • Genetic variability in plant height, number of leaves and seedling diameter in salinity tolerant sugarcane genotype CoM-265 and salinity susceptible sugarcane genotype CoC-671 were delineated by Peroxidase, esterase and alcohol dehydrogenase isozymes pattern and leaf soluble protein levels. • The reduction in plant height, number of leaves and seedling diameter was less in salinity tolerant sugarcane genotype CoM-265 as compared to salinity susceptible sugarcane genotype CoC-671 at different salinity levels, i.e., 0.41, 2.31, 4.21 and 8.01 dSm -1 . Plant salt tolerance was analyzed based on the relative plant growth rate and the plant survival rate after exposure to a range of salt concentrations. The salinity tolerance limits of sugarcane ranges from 1.7 to 2.3 dSm -1 . • Understanding genetic relationship between sugarcane genotypes CoM-265 (salinity tolerant) and CoC-671 (salinity susceptible) would aid in breeding programs aimed at widening the genetic basis in sugarcane. • Present study would provide the genetic basis to characterize and identify the sugarcane genotypes suitable for growing in saline environment. The salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 were evaluated for Peroxidase (POX), Esterase (EST) and Alcohol Dehydrogenase (ADH) isozymes and soluble protein profiling by SDS and native-PAGE at salinity levels 0.41 dSm -1 , 2.31 dSm -1 , 4.21 dSm -1 , and 8.01 dSm -1 maintained by NaCl solution. The plant height, number of leaves and seedling diameter got reduced in salinity susceptible sugarcane genotype CoC-671 as well as salinity tolerant sugarcane genotype CoM-265 with increase in salinity levels. However, reduction in plant height, number of leaves and seedling diameter was less in salinity tolerant sugarcane genotype CoM-265 as compared to salinity susceptible sugarcane genotype CoC-671. The POX isozyme profiling revealed that salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 had variation in soluble protein band intensity at different salinity levels with relative mobility (Rm) 0.137. The present study could be useful for genetic variability analysis in sugarcane genotypes differing in salinity stress tolerance capability.
Source: Biotechnology Reports
Authors: Manisha Rameshrao Patil, A.A. Kale, Ajay Kumar Singh, Priyanka Rameshrao Patil, Shaheen Badshah Inamdar, R.D. Satbhai
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $3,480
OpenAlex ID: W4407158109