Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model
Year: 2025
Type: article
Abstract: The lack of an etiologic Parkinson’s disease (PD) model in non-human primates has inhibited our understanding of the disease and the development of therapies. Here, we present a protocol for generating a PD model in Macaca fascicularis . We describe steps for localizing the substantia nigra and striatum using magnetic resonance imaging (MRI). We then detail a technique for the intracerebral administration of adeno-associated virus (AAV)-mediated overexpression of α-synuclein into the substantia nigra plus the injection of poly(ADP-ribose) (PAR) into the striatum. For complete details on the use and execution of this protocol, please refer to Liu et al. 1 • Steps for the injection of AAV-α-syn into the substantia nigra • Instructions for the injection of poly(ADP-ribose) into the striatum • Guidance on the location of brain nuclei by MRI image • Procedures for brain mapping surgery in monkeys Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The lack of an etiologic Parkinson’s disease (PD) model in non-human primates has inhibited our understanding of the disease and the development of therapies. Here, we present a protocol for generating a PD model in Macaca fascicularis . We describe steps for localizing the substantia nigra and striatum using magnetic resonance imaging (MRI). We then detail a technique for the intracerebral administration of adeno-associated virus (AAV)-mediated overexpression of α-synuclein into the substantia nigra plus the injection of poly(ADP-ribose) (PAR) into the striatum.
Source: STAR Protocols
Authors: Shuyi Liu, Wei Si, Zhengbo Wang
Institution: Kunming University of Science and Technology
Language: English
Subfield: Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,550
Funders: National Natural Science Foundation of China, Natural Science Foundation of Yunnan Province, National Key Research and Development Program of China, Cultivating Plan Program for the Leader in Science and Technology of Yunnan Province, National Key Research and Development Program of China Stem Cell and Translational Research
OpenAlex ID: W4407190618