Work

Interferon regulatory factor -9 in Labeo rohita, an insight into its interacting domain residues and dynamic responses to PAMPs stimulation and pathogenic challenges

Year: 2025

Type: article

Abstract: • LrIRF9 cDNA contains a DNA binding domain (DBD) and IRF3-transactivation domain. • Nucleotide-binding amino acid residues in the DBD include H44, R83, R85, C86 and N89. • Peptide binding residues in the IRF3 domain lie between Y243-K330. • LrIRF9 gene responds against LPS and poly I: c stimulation and bacterial infection. Immune response to viral infection involves the intricate regulation of type I interferons (IFNs) and interferon-stimulated genes (ISGs) controlled by the interferon regulatory factors (IRFs). Although IFN signaling and the role of IRFs in its regulation have been considerably researched in higher vertebrates, their influence on piscine immunity remains largely unexplained. This article describes the cloning, characterization, and 3D modeling, of Labeo rohita IRF9 (LrIRF9) in depicting the presence of highly conserved DNA-binding domain (DBD) and IRF3-transactivation domains. In the DBD, the predicted nucleotide-binding residues include H 44 , R 83 , R 85 , C 86 and N 89, while peptide binding residues in the IRF3-transactivation domain involve Y 243 , K 288 , L 289 , L 290 , M 293 , K 294 , Q 313 , G 314 , F 317 , and K 330 . Phylogenetically, LrIRF9 is evolutionarily close to IRF9 of Cyprinus carpio (93.5 %), Ctenopharyngodon idella (88.2 %) and Mylopharyngodon piceus (88 %). The LrIRF9 gene is expressed in all examined organs, with notably higher levels detected in the intestine, skin, and blood compared to other tissues. In-vitro and in-vivo stimulation with poly I: C or LPS significantly ( p < 0.05) enhanced LrIRF9 gene expression in most of the examined tissues, RBCs, PBLs, and macrophages. In response to Aeromonas hydrophila and Edwardsiella tarda infection, LrIRF9 gene expression was also enhanced in some of the tested organs/tissues. Collectively, these data provide a critical insight into the piscine IRF9 during pathogenic insults.

Source: Comparative Immunology Reports

DOI: 10.1016/j.cirep.2025.200209

Authors: Bristy Ganguly, Smruti Mahapatra, Saswati Pani, Rakesh Das, Ashis Saha, Mrinal Samanta

Institutions: Indian Council of Agricultural Research, Central Institute of Freshwater Aquaculture

Language: English

Topic: interferon and immune responses

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,800

Funder: Department of Biotechnology, Ministry of Science and Technology, India