Work

Characterisation of betanodavirus of finfishes from India and its purification using CsCl density gradient ultracentrifugation

Year: 2024

Type: article

Abstract: Betanodavirus is a pathogen of many tropical and temperate marine and brackishwater fish species. It is an etiological agent of viral nervous necrosis (VNN) in fish larvae and juveniles, leading up to 100% mortality. One among the best and effective approaches to control viral diseases in aquaculture is vaccination. Purified viral particles are useful for the development of immunodiagnostics and vaccines. In this study, purification of betanodavirus was attempted through ultracentrifugation using CsCl density gradient. SSN-1 cells were infected with betanodavirus and after recording cytopathic effect (CPE), cells were harvested. Virus infection was confirmed by PCR assay using reported primers and sequencing. Transmission electron microscopy (TEM) was carried out for confirmation ofinfection. Virus from cell culture supernatants was purified using CsCl density gradient and ultracentrifugation. Purified virus was further confirmed by PCR assay, SDS-PAGE analysis and TEM. The purified particles were tested for infectivity by incubating with SSN-1 cell line for its biological activity. Keywords: Betanodavirus, CsCl gradient, Density gradient, SSN-1 cells, Ultracentrifugation TEM, VNN

Source: Indian Journal of Fisheries

DOI: 10.21077/ijf.2024.71.4.125472-12

Authors: G. S. Ghode, M. Makesh, R. Bharathi Rathinam, Gayatri Tripathi

Institutions: Indian Council of Agricultural Research, Central Institute of Fisheries Education, Central Institute of Brackishwater Aquaculture

Language: English

Topic: Aquaculture disease management and microbiota

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Indian Council of Agricultural Research