Work

Taguchi L9 Optimization of Microwave‐Assisted Solid–Liquid Duo Heating for Berberine Extraction With HPTLC Analysis

Year: 2025

Type: article

Abstract: We present an optimized approach for the extraction of berberine using microwave-assisted solid-liquid duo heating. The conventional procedure relies on the simultaneous heating of the sample matrix and the dissolving solvent under microwave energy. To improve the efficiency of this process, we employed the Taguchi L9 orthogonal design methodology, focusing on key extraction parameters, including microwave power, irradiation time, solute-solvent ratio, and temperature. Berberine content was quantified using high-performance thin-layer chromatography (HPTLC). Our findings revealed that the most influential parameters in the berberine extraction process were microwave power (ranked as the most critical factor), followed by irradiation time (second in importance), and temperature (third in significance). Utilizing the dual heating approach in microwave-assisted extraction (MAE) led to a more precise and efficient determination of extraction parameters, resulting in enhanced accuracy and higher yield. Additionally, this optimized method significantly reduced the overall extraction time, making it an ideal choice for berberine extraction under optimal conditions.

Source: Biomedical Chromatography

DOI: 10.1002/bmc.70008

Authors: Saurabh Satija, Manish Vyas, Parijat Pandey, Munish Garg

Institutions: University of Wollongong, Lovely Professional University, Gurugram University, Maharshi Dayanand University

Language: English

Topic: Berberine and alkaloids research

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $3,710

Funders: University Grants Commission, University of Wollongong