Nematicidal potential of Microbacterium maritypicum Sneb159 against Heterodera glycines and the complete genome sequence analysis
Year: 2025
Type: article
Abstract: Introduction Heterodera glycines is one of the most important pathogens of soybean production worldwide. Biological control provides a strategy for sustainable and environmentally friendly nematode management. Methods In this study, solid-phase microextraction gas chromatography-mass spectrometry (SPME-GC-MS) was used to reveal the volatile nematicidal compounds of Microbacterium maritypicum Sneb159 and the mode of action was further elucidated via whole genome sequencing. Results The present study demonstrated that M. maritypicum Sneb159 fermentation broth showed strong nematicidal activities against H. glycines . The filtrate rather than bacterial cells played a role in the nematicidal character, and reduced the invasion number as well as suppressed the development of juveniles in soybean. The analysis of chemotaxis showed that M. maritypicum Sneb159 has a repellent effect on H. glycines in pot experiments. The volatiles produced by M. maritypicum Sneb159 are toxic to H. glycines for both juveniles and eggs. The seven compounds were analyzed using SPME-GC-MS. In the bioassays, dimethyl disulfide and dimethyl trisulfide showed both direct contact and fumigated effect on juveniles and eggs. The complete genome sequence of the bacterium M. maritypicum Sneb159 was completed using the PacBio sequencing platform. The genome comprised 3895529 bp and a 68.63% G + C content. Three secondary metabolites gene clusters were predicted by the antiSMASH system. Discussion The findings reveal multifunction of M. maritypicum Sneb159 towards H. glycines , and has the potential to be developed as a safe nematicidal agent.
Source: Frontiers in Plant Science
Authors: Jing Zhao, Zhifu Xing, Aatika Sikandar, Yuxi Duan
Institutions: Shenyang Agricultural University, Hainan University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W4407262188