Synthesis of Black Turmeric Extract Nanoparticles (curcuma Caesia) and Its Cytotoxic Activity on T47d Cells
Year: 2024
Type: article
Abstract: Objective: This study aimed to formulate black turmeric into nanoparticle preparations with various concentrations of chitosan and determine its cytotoxic effect on T47D breast cancer cells. Methods: Extraction was carried out by the maceration method. Black turmeric condensed extract was formulated into nanoparticles using the ionic gelation method, which was a method that relies on the cross-linking agent sodium tripolyphosphate (Na-TPP). The cytotoxic activity of black turmeric extract was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. Results: The results showed that black turmeric extract nanoparticles have a size range of 266-558 nm and were positively charged with zeta potential values ranging from 3.3 to 9.7 mV. The encapsulation efficiency of black turmeric extract nanoparticles was 63.42%. The results of the cytotoxic test showed that both black turmeric extract and black turmeric extract nanoparticles showed moderate cytotoxic activity, with the IC50 values of the two preparations were 78.60 µg/ml and 162.95 µg/ml, respectively. Conclusion: The results obtained in this research indicate a promising potential of nanoparticles of black turmeric extract as a cytotoxic agent for the treatment of breast cancer.
Authors: MUHAMMAD DA’I, NUR AZIZAH, ANDREA Y RAHMANA, SETYO NURWAINI, ERINDYAH R WIKANTYASNING
Language: English
Subfield: Molecular Medicine
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4407269603