Work

Deciphering key parameters enhancing lentiviral vector producer cells yields: Vector components copy number and expression

Year: 2025

Type: article

Abstract: -complementation with each genetic cassette further supported this conclusion. The producer LV clones expressed constitutively the 4070A envelope, but the overexpression of the VSV-G envelope increased 30-fold the titer supporting the envelope as key determinant in LV quality. This work further elucidates bottlenecks in LV producer cell line development providing insights for their optimization.

Source: Molecular Therapy — Methods & Clinical Development

DOI: 10.1016/j.omtm.2025.101431

Authors: Ana S. Formas-Oliveira, Mariana Valentim Ferreira, Ana Sofia Coroadinha

Institutions: Instituto de Biologia Experimental e Tecnológica, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,900

Funders: Ministério da Ciência, Tecnologia e Ensino Superior, Fundação para a Ciência e a Tecnologia