Work

Determination of protein transporter function using Raman spectroscopy

Year: 2025

Type: article

Abstract: , which is shown to transport alkyne-labelled ATP molecules (N6pATP), which was readily detected using Raman spectroscopy. We show that ATP transport can be detected in a time-dependent manner using alkyne labels and demonstrate the substrate specificity of the transporter for purine but not pyrimidine substrates. This work establishes that Raman spectroscopy is an excellent alternative to using radioactive substrates in analysing, not only pathogen transporters, but potentially any transporter in which its substrate can be alkyne tagged.

Source: Microbiology

DOI: 10.1099/mic.0.001526

Authors: Dominic Gilchrist, Meez Islam, Muhammad Safwan Akram, Paul Dean

Institution: Teesside University

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funder: Teesside University

OpenAlex ID: W4407318557

FWCI:

Cites: 20

Cited by:

Related to: 10