Work

Sample storage and fasting times affect blood serum and plasma components in fasted and non-fasted broiler chickens

Year: 2025

Type: article

Abstract: We determined the impacts of blood fraction, fasting and samples storage times on the total protein, albumin, globulin, uric acid, creatinine concentrations, and alkaline phosphatase activity in 70 45- d -old male Cobb 500® broiler chickens. Blood was collected at 2-h intervals (0, 2, 4, 6, 8, 10, and 12 h). Serum and plasma fractions were stored at -20°C and analyzed on 0, 30, and 60 days. Serum and plasma uric acid concentrations increased with increasing storage time, while a reduction in creatinine concentration and serum alkaline phosphatase activity was observed. Serum creatinine and alkaline phosphatase showed a quadratic effect, with a peak concentration of 0.208 mg dL -1 at 4 h and 32 min of fasting, and the lowest concentration of 2933 U l -1 at 8 h of fasting, respectively. Plasma total protein, albumin, and creatinine showed a quadratic effect, with a peak concentration of 35.46 g l -1 at 7 h and 48 min, 15.36 g l -1 at 10 h and 48 min, and 0.18 mg dL -1 at 7 h and 46 min, respectively. Overall, serum and plasma can be used for the analysis of total protein, albumin and globulin, with a fasting time of 12 h and storage for up to 60 days at -20°C. Both serum and plasma fractions can be used for uric acid analysis, but immediate analysis after sample collection is recommended, and a 12-h fast can be used. It is recommended to use serum for creatinine and alkaline phosphatase analysis, with storage for up to 30 days at -20°C.

Source: The Journal of Applied Poultry Research

DOI: 10.1016/j.japr.2025.100525