Year: 2020
Type: article
Abstract: The goal of the research was to study the protein content and its amino acid composition in the seeds of soybean variety samples (lines) obtained by the somaclonal variability method in culture in vitro using of cadmium ions in nutrient media. Somaclonal lines were obtained in the breeding nursery of Federal Research Center for Agrobiotechnologies of the Far East named after A.K. Chaika in accordance with the agrotechnics adopted for Primorskiy Krai in 2017–2018. Studies of the biochemical composition of soybean seeds were carried out at the All-Russian Scientific Research Institute of Soybean Test Laboratory. Variety samples were analyzed for protein content and its amino acid composition by the near-infrared spectroscopy method using “FOSS NIR Systems 5000” analyzer. On the basis of the obtained data, we found lines with a significant excess of the standard level concerning the following characteristics: protein content by 5.3–9.5%, histidine – by 56–82%, leucine – by 3.8–5.0%, and isoleucine – by 18%. As a result of cluster analysis, three groups (subclusters) of regenerants were singled out, which differ in a complex of biochemical features with improved characteristics. The lines of the first group (R1590, R1583) are characterized by high protein content – 40.4–42.2%. The second group is represented by lines (R1597, R1569) combining a high content of protein (39.9–40.3%) and histidine (9.0–9.1%). The third one combines the middle-protein group of somaclones (R1606, R1567, R1585) with an increased content of histidine (7.8–8.7%) and leucine (8.3–8.4%).
Source: Far Eastern Agrarian Herald
Authors: Galina A. Kodirova, Galina V. Kubankova, Sergey E. Nizkii, Pyotr V. Fisenko
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W4407346393