Work

Extraction Optimization and Bioactivity of Polysaccharides from Ganoderma leucocontextum Spores

Year: 2025

Type: article

Abstract: Background: Oxidative stress is associated with the occurrence and progress of aging. Natural polysaccharides have attracted considerable attention in the field of antioxidants and anti-aging products due to their superior biological activity and low toxicity. Ganoderma leucocontextum is primarily found in the Tibetan plateau region and is classified as a subspecies of Ganoderma. Known as the famous white Ganoderma, it is a precious food and medicine that has potent biological activity, including antitumor, hypoglycemic, and immune regulation. Since available resources are limited, there are few studies on the spore of Ganoderma leucocontextum. Methods: In this work, a polysaccharide (named GLSP) was extracted from the spore of Ganoderma leucocontextum using a fast, simple, efficient, and environmentally friendly extraction process: the three-phase partitioning (TPP) method. Results: The extraction condition was optimized under the Box–Behnken design (BBD): ratio of the solute to the solvent, 1:21.126 (w/v); (NH4)2SO4 concentration, 30% (w/v); ratio of the slurry to tert-butanol, 1:1.945 (v/v); and shaking temperature, 54.136 °C. Furthermore, a polysaccharide termed GLSP-A1 was purified from GLSP by column chromatography. The basic physicochemical properties were analyzed by molecular weight, Fourier transform infrared spectroscopy, monosaccharide composition, and scanning electron microscopy. Conclusions: GLSP-A1 down-regulated the expression of the pro-inflammation cytokines interleukin-6 and interleukin-1β, indicating favorable in vitro anti-inflammatory properties. In vivo, the effect of GLSP-A1 on aging was examined using the Caenorhabditis model. The results showed that GLSP-A1 reduced reactive oxygen species levels and lipofuscin accumulation. In general, these findings improve our understanding of the chemical content and bioactivity of a polysaccharide from Ganoderma leucocontextum spore and highlight the possibility of GLSP-A1 being utilized in dietary supplements for its anti-aging properties.

Source: Pharmaceuticals

DOI: 10.3390/ph18020241

Authors: Siying Peng, Yanghui Ou, Yali Zhang, Hongliang Yao, Wen-Hua Chen

Institutions: Guangdong Academy of Sciences, Wuyi University, Wuyi University

Language: English

Topic: Fungal Biology and Applications

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,499