Work

Optimization of Mexican Oregano Essential Oil (Lippia Graveolens) Extraction with Fungicidal and Oomyceticidal Effects Against Plant Pathogens

Year: 2025

Type: article

Abstract: Botanical extracts are being considered for integration into disease management programs to control plant pathogenic fungi and oomycetes. A promising extract with potential is the essential oil of Lippia graveolens. However, its extraction process has not been optimized. Since optimizing process conditions can impact fungicidal and/or oomyceticidal effects, this research implemented time, temperature, and solid/liquid ratio conditions to maximize the fungicidal and oomyceticidal effects. The effectiveness was evaluated through parameters of mycelial growth inhibition, spore germination inhibition, minimum inhibitory and fungicidal/oomyceticidal concentration for the fungi Gilbertella persicaria, Agroathelia rolfsii, and Colletotrichum gloeosporioides, as well as the oomycete Phytophthora capsici. Optimal conditions were as follows: time: 46.27 min; temperature: 329.34 °C; and solid/liquid ratio: 80.35 g/L. In general, the optimized treatment was more effective in A. rolfsii ≥ P. capsica > G. persicaria ≥ C. gloeosporioides in all assays. These results demonstrate the fungicidal and oomiceticidal effects of L. graveolens essential oil, with potential for commercial product formulation.

Source: Processes

DOI: 10.3390/pr13020490