Dynamic Activation of NADPH Oxidases in Immune Responses Modulates Differentiation, Function, and Lifespan of Plasma Cells
Year: 2025
Type: article
Abstract: ABSTRACT NADPH‐oxidase (NOX)‐derived reactive oxygen species (ROS) have been described to play essential roles in B‐cell activation processes. However, several key questions concerning NOX activity and subsequent ROS production remain unaddressed, including fundamental processes such as differentiation, functional competence, cellular metabolism, and viability. This study investigated these questions in a murine B‐cell response after secondary immunization. We combined single‐cell transcriptomics and single‐cell detection of NOX activity and observed that various subsets of B cells dynamically express NOX1 and NOX2 . The NOX + cellular phenotype correlated with increased activity of metabolic pathways, augmented lactate production, lower IgG secretion rates, and markers for longevity. The NOX + cellular phenotype was also associated with increased cellular stress and apoptosis, underscoring the intricate relationship between ROS and cellular survival. Consequently, these insights advance our understanding of how long‐lived humoral immunity is formed.
Source: European Journal of Immunology
Authors: Olivia T. M. Bucheli, Daniela Rodrigues, Carolin Ulbricht, Anja E. Hauser, Klaus Eyer
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,550
Awards: High-throughput single-cell phenotypic analysis of functional antibody repertoires, FOR 5560: Wechselwirkungen zwischen dem Stoffwechsel und der Signalübertragung in BZellen, G2219446283, Charakterisierung des Einflusses der Mikroumgebung von Plasmazellen auf ihre Funktion, Functionally targeted single-cell sequencing - Applications for antibody discovery, G7006139384, G72142182, G7944295140, G8690818240
OpenAlex ID: W4407388441