Figure 5 from A Melanoma Brain Metastasis CTC Signature and CTC:B-cell Clusters Associate with Secondary Liver Metastasis: A Melanoma Brain–Liver Metastasis Axis
Year: 2025
Type: other
Abstract: RNA-seq analyses of blood and brain from second-generation huNBSGW and NBSGW mice. A and B, CTCs from blood of second-generation huNBSGW CDXs (N = 3) were harvested via Parsortix, followed by RNA-seq interrogation. Distinct unsupervised hierarchical clustering of gene expression between huNBSGW CTCs and uninjected CTC-derived clonal cells (A) or blood-derived CTCs from immunodeficient NSG mice (B). C, RNA-seq analyses of cells isolated from MBM cells of second-generation huNBSGW/NBSGW CDXs with heat-maps showing differential clustering of MBM-derived cells in the presence or absence of human immune cells. Bottom, Top molecular pathways resulting from comparisons in huNBSGW vs. NBSGW CDXs (Reactome pathway database). All RNA-seq analyses showed the presence of B cell–related pathways in huNBSGW CDXs (highlighted in yellow). See “Materials and Methods” for experimental details. MBM, melanoma brain metastasis.
Language: English
Topic: Cancer Cells and Metastasis
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4407411117