Association of HLA-B51/5 in Patients of Behcets Disease Referred to a Tertiary Care Hospital in Pakistan
Year: 2025
Type: article
Study design: Observational Study
Abstract: OBJECTIVE: To determine the association of HLA-B51/5 in patients of Behcet's disease (BD) in local symptomatic patients. STUDY DESIGN: A cross-sectional study. Place and Duration of the Study: Department of Immunology, Armed Forces Institute of Pathology, Rawalpindi, Pakistan, from August 2020 to July 2023. METHODOLOGY: Blood samples of 100 study participants, including 50 symptomatic patients of BD and 50 disease-free individuals (healthy controls), were taken. DNA was extracted and amplified using sequence-specific primers. Electrophoresis was conducted on 2% agarose gel to separate the DNA. The presence of HLA-B51/5 alleles was determined by analysing the unique band patterns visible on the gel under the ultraviolet light. Descriptive statistics were shown as mean ± SD. Chi-square test was employed to find any significant association between HLA-B51/5 and the disease group and its severity sub-groups. A p-value of less than 0.05 was deemed significant. RESULTS: The majority of participants, 73 (73%), were females and 27 (27%) were males, with a mean age of 26.84 ± 5.31 years. HLA-B51/5 was positive in 33 of the BD patients (66.0%, <0.001) and 4 of the healthy controls (8.0%). There was no significant difference in HLA-B51 among mild, moderate, and severe BD sub-groups. CONCLUSION: HLA-B51/5 is strongly associated with BD in local population and serves as a useful tool for diagnosis of BD. However, it is not associated with the disease severity. KEY WORDS: Agarose gel, Behcet's disease, Electrophoresis, HLA-B51/5 allele, ICBD criteria.
Institution: Armed Forces Institute of Pathology
Language: English
Subfield: Ophthalmology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4407416741