RNA m5C methylation mediated by Ybx1 ensures hematopoietic stem and progenitor cell expansion
Year: 2025
Type: article
Abstract: Hematopoietic stem and progenitor cells (HSPCs) undergo rapid transcriptional transitions among distinct cell states and functional properties during development, but the underlying molecular mechanism is largely unknown. Here, we characterize the mRNA m 5 C landscape of developing HSPCs in zebrafish and found that m 5 C modification is essential for HSPC expansion through maintaining mRNA stability. Deletion of the m 5 C reader, Y-box binding protein 1 (Ybx1), significantly inhibits the proliferation of HSPCs in zebrafish and mice. Mechanistically, Ybx1 recognizes m 5 C-modified mRNAs and maintains the stability of cell-cycle-related transcripts, thereby ensuring proper HSPC expansion. This study reveals the critical role of Ybx1-mediated mRNA m 5 C modification in developmental hematopoiesis and provides new insights and epitransciptomic strategies for optimizing HSPC expansion.
Source: Cell Reports
Authors: Fan Liu, Fan Liu, Mengke Wang, Suwei Gao, Gege Song, Mengyao Liu, Ying Li, Piao Sun, Weiyi Lai, Hailin Wang, Yun-Gui Yang, Feng Liu, Feng Liu, Ying Yang, Lu Wang
Institutions: Shandong University, Chinese Academy of Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Institute of Hematology & Blood Diseases Hospital, University of Chinese Academy of Sciences, Institute of Zoology, State Key Laboratory of Membrane Biology, State Key Laboratory of Experimental Hematology, Beijing Institute of Genomics, Research Center for Eco-Environmental Sciences, State Key Laboratory of Environmental Chemistry and Ecotoxicology
Language: English
Topic: RNA modifications and cancer
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,620
OpenAlex ID: W4407479154