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Investigating Polyhydroxyalkanoate Synthesis for Insights into Drug Resistance in Xanthomonas oryzae pv. oryzae

Year: 2025

Type: article

Abstract: Polyhydroxyalkanoates (PHAs), synthesized by Xanthomonas to endure adverse conditions, are primarily regulated by the critical genes phaC and phaZ. Poly-3-hydroxybutyrate (PHB), a common polyhydroxyalkanoate (PHA), has been implicated in metabolism, pathogenicity, and various physiological processes in Xanthomonas oryzae pv. oryzae (Xoo). In this study, we investigated the effects of HN-2 using n-butanol extract (HN-2 n-butanol extract) derived from Bacillus velezensis on Xoo. The results showed that HN-2 n-butanol extract could induce PHB accumulation in Xoo, potentially via surfactin. Moreover, examination of drug resistance, pathogenicity, and morphological characteristics of Xoo revealed PHB played a significant role in the drug resistance, pathogenicity, membrane integrity, and growth rate of Xoo strains following the deletion of phaZ and phaC. The ∆phaZ strain was the most significant, with a growth rate reduced to 58.19% of the PXO99A at 36 h and an inhibition zone 57.46% larger than that of PXO99A by HN-2 n-butanol extract. Transmission electron microscopy further revealed blank spots in Xoo after treatment, with the fewest spots observed in ∆phaZ, indicating its impaired ability to repair and maintain membrane integrity. These findings offer valuable insights that could serve as a foundation for elucidating the mechanisms of drug resistance and future research on preventing Xoo-induced diseases.

Source: International Journal of Molecular Sciences

DOI: 10.3390/ijms26041601