Year: 2025
Type: article
Abstract: Limb loss resulting from disease or trauma affects an estimated 185,000 Americans annually, significantly reducing their quality of life. Consequently, successful attempts to regrow missing appendages could substantially improve the prognosis for amputees. In mice, the digit tip spontaneously regenerates resected tissues following distal amputation, whereas this capacity diminishes at more proximal levels after amputation. Moreover, regenerative potential is influenced by genetic variations among inbred mouse strains: LG/J (healer) mice exhibit superior reparative potential compared to SM/J (non-healer) mice. This study investigated the response to various levels of digit amputation in these mice to determine whether this strain-dependent healing response translates to the regeneration of complex tissues. Evaluation of skeletal regrowth, cell proliferation, and differential gene and protein expression reveals that digit regeneration is more robust in LG/J mice compared to SM/J mice at multiple amputation levels, suggesting that the regenerative capacity of composite tissues is genetically heritable in mice.
Source: npj Regenerative Medicine
Authors: Feini Qu, Kristin L. Lenz, Gwendalyn L. Krekeler, Xin Duan, Muhammad Farooq Rai, Farshid Guilak
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,190
Funders: U.S. Department of Health and Human Services, University of Washington, Shriners Hospitals for Children, National Institutes of Health, National Institute on Aging, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: Deconstructing Cartilage Mechanotransduction by Piezo Channels, G1699998815, Genetically-engineered stem cells for self-regulating arthritis therapy, VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS, G222266920, G2271249105, G2366089456, G2472603119, G2775599718, G291369353, G2925877155, G3519473169, Mechanisms of Skeletal Morphogenesis During Digit Tip Regeneration, G3810130841, G3895366428, G4619719930, G4650882932, G5039167063, G5330480267, G5367714306, G5588909404, G5753973162, OBESITY, BIOMECHANICS, AND INFLAMMATION IN OSTEOARTHRITIS, Resource Based Center for Musculoskeletal Biology and Medicine (Overall Application), G6702224243, Cellular and Molecular Mechanisms of Murine Digit Regeneration, G6950686347, G7008996325, G8118544813, G812923891, G8307755323, G8320478738, G8487592227, G8551615088, G8694065446, G8840517274, G8922727010, G9000108560, G9000110435, G9000114129, G9000197611, G9000217772, G9000308115, Washington University Rheumatic Diseases Research Resource-based Center
OpenAlex ID: W4407570573