Work

Generation of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC double reporter line for monitoring of pancreatic differentiation

Year: 2025

Type: article

Abstract: Pancreatic islets consist of several different endocrine cell types that work in harmony. Aside from primary pancreatic islets, stem cell-derived pancreatic islets can be used as an alternative research and disease model. Here, we introduce a double reporter line of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC through CRISPR/Cas9-mediated insertion of mCherry in the C-terminus of C-Peptide in the previously published ARX-CFP hiPSC line. This reporter line allows live monitoring of stem cell-derived pancreatic alpha and beta cells throughout differentiation.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2025.103685

Authors: Eunike Sawitning Ayu Setyono, Nicole Katarina Rogers, Anita Hofmann, Heiko Lickert, Ingo Burtscher

Institutions: Helmholtz Munich, German Center for Diabetes Research, Technical University of Munich

Language: English

Topic: Pancreatic function and diabetes

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,360