Year: 2025
Type: article
Abstract: Background: Significant progress has been made in understanding the mechanisms of psoriasis, particularly the role of the interleukin (IL)-23/T-helper (Th) 17 axis, leading to novel, targeted therapies. However, many patients develop resistance to treatment over time. Thus, exploring new therapeutic strategies for severe refractory psoriasis remains crucial. Objective: To investigate the effect of cladribine on imiquimod induced psoriasiform dermatitis in mice. Methods: We established an imiquimod (IMQ)-induced psoriasiform dermatitis mouse model to investigate cladribine's effects on skin immune cells. Mice were allocated to five groups: Control, IMQ, High-dose cladribine (30mg/kg), Low-dose cladribine (20mg/kg), and Methotrexate. We assessed cumulative scores, skin pathology, immunohistochemistry, flow cytometry, and serum cytokines. We also studied cladribine's long-term efficacy by reapplying IMQ for a second round (7 days) after five half-lives of cladribine. Results: Cladribine significantly ameliorated symptoms and pathological features of IMQ-induced psoriasis in both high and low-dose groups, with efficacy comparable to methotrexate. Cladribine dose-dependently reduced Th17 and Th1 cell frequencies in psoriatic skin, along with associated cytokines. High-dose cladribine demonstrated sustained inhibition of IMQ-induced psoriasis. Conclusion: These findings indicate that cladribine can ameliorate imiquimod-induced psoriasiform dermatitis in mice, exhibiting a dose-dependent and sustained therapeutic effect.
DOI: 10.2147/ccid.s511351
Authors: Jingwen Xue, Dan Shu, Yi Zhao
Institutions: Beijing Tsinghua Chang Gung Hospital, Tsinghua University
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,836
Awards: G1577781108, G730532320
OpenAlex ID: W4407669145