Work

Rb1 restores palmitic acid-induced reduction of Ca2+ influx by activating PLC in EA cells and PLD in MOVAS cells

Year: 2025

Type: article

Abstract: Recent interest has focused on the role of Ca 2+ in regulating health problems, including cardiovascular disease and colorectal cancer. The inverse correlation between colon cancer and serum Ca 2+ underlines the importance of understanding intracellular Ca 2+ dynamics. Studies are also evaluating the contributions of abnormalities in Ca²⁺ homeostasis and intracellular dysfunction to the pathogenesis of metabolic syndrome as a precursor of cardiovascular disease. In this study, we investigated the changes of Ca 2+ dynamics and ginsenoside Rb 1 (Rb 1 )-induced recovery in two vascular cell lines exposed to palmitic acid (PA), the most abundant active ingredient in palm oil. The mechanism underlying the Rb 1 -induced recovery was examined in a store operated Ca 2+ entry model by Ca 2+ store depletion. PA reduced the Ca 2+ influx in both EA.hy926 (EA) and MOVAS cells, and this change was restored by Rb 1 . In EA cells, the Rb 1 -induced restoration was abolished by U73122 or 2-APB. In MOVAS cells, meanwhile, the effect of Rb 1 was abolished by FIPI, U73122 and U73343. Under normal conditions, Rb 1 itself altered phospholipid signaling (PLC in EA cells and PLD in MOVAS cells), but did not affect Ca 2+ homeostasis. These differences resulted in differences in downstream actions, as KB-R7943 and nifedipine inhibited Rb 1 -mediated Ca 2+ influx recovery only in MOVAS cells. In conclusion, Rb 1 rescues the PA-induced Ca 2+ influx by appropriately activating PLC in EA cells and PLD in MOVAS cells. This demonstrates that Ca 2+ dynamics are elaborately regulated via intracellular Ca 2+ signaling networks, suggesting a potential strategy for maintaining vascular Ca 2+ homeostasis in hyperlipidemic environments. • Palmitic acid induced decrease of Ca 2+ influx in EA and MOVAS cells. • Rb 1 restores palmitic acid-induced decrease of Ca 2+ influx by activating PLC in EA cells. • Rb 1 restores palmitic acid-induced reduction of Ca 2+ influx by activating PLD in MOVAS cells.

Source: Biomedicine & Pharmacotherapy

DOI: 10.1016/j.biopha.2025.117927

Authors: In-Young Choi, Yoo Jin Kim, So Young Kim, Min Kyung Lee, Geun Hee Seol

Institution: Korea University

Language: English

Topic: Ginseng Biological Effects and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,610