Work

Leukopenia, weight loss and oral mucositis induced by 5-Fluorouracil in hamsters’ model: A regenerative approach using electrospun poly(Lactic-co-Glycolic Acid) membrane

Year: 2025

Type: article

Abstract: // Ana Chor 1 , 4 , * , Hélio dos Santos Dutra 2 , Marcos Lopes Dias 3 , Raquel Pires Gonçalves 3 , 4 , Alexandre Malta Rossi 4 , Christina Maeda Takiya 5 , * and Marcos Farina 1 1 Biomineralization Laboratory, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil 2 University Hospital, Bone Marrow Transplantation Unit, Federal University of Rio de Janeiro, Rio de Janeiro, 21941-902, Brazil 3 Catalysis Laboratory for Polymerization, Recycling and Biodegradable Polymers, Institute of Macromolecules Professor Eloisa Mano, Federal University of Rio de Janeiro, Rio de Janeiro 21941-598, Brazil 4 Department of Condensed Matter, Applied Physics and Nanoscience, Brazilian Center for Research in Physics, Rio de Janeiro 220-180, Brazil 5 Immunopathology Laboratory, Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro 20941-902, Brazil * These authors contributed equally to this work Correspondence to: Ana Chor, email: [email protected] Keywords: leukopenia; weight loss; oral mucositis; PLGA dressing; regeneration Received: February 05, 2024 Accepted: December 09, 2024 Published: February 18, 2025 Copyright: © 2025 Chor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ABSTRACT Clinical parameters of leukogram and weight were analyzed in animal models before and after seven days of 5-FU infusions. A comparison of leukograms before and after 5-FU administrations was analyzed. The results showed a significative difference ( p = 0,004), confirming the immunosuppression. There was a decrease in the weight of the animals after 7 days of 5-FU infusions ( p = 0.02). After immunosuppression, oral mucositis (OM) ulcerative lesions were established. Two of ten animals were aleatory selected to receive PLGA dressings. The electrospun PLGA membranes with or without autologous cells were applied to the ulcerative lesions, aiming to accelerate the regeneration process. Although this therapeutic innovation for OM lesions was still not tested in the bioengineering area, morphological analysis presented promising results. Lesions covered by cell-free PLGA exhibited areas of inflammation persistence and angiogenesis, and cell-seeded PLGA membrane exhibited complete reepithelialization after 6 days, with minor inflammatory infiltrate. Interestingly, the present work showed preclinical parameters of cachexia induced by chemotherapy for cancer treatment. Moreover, it showed an innovative approach by applying dressings constituted of electrospun PLGA with the addition of autologous mesenchymal cells for OM ulcerative lesions. This promising innovation will pave the way for future applications in oral mucosa lesions.

Source: Oncotarget

DOI: 10.18632/oncotarget.28685