Work

Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4

Year: 2025

Type: article

Abstract: suppression, host cell apoptosis induction, and PRRSV detection facilitation. In response to wild or attenuated PRRSV strains, antibodies against non-structural proteins are generated, while inactivated vaccines fail to elicit such responses. Employing the Enzyme-Linked Immunosorbent Assay (ELISA) method targeting non-structural proteins helps discern the immune effects of inactivated versus wild or attenuated vaccine strains. The study focused on the NSP4 protein from the PRRSV XH-GD strain (GenBank No. EU624117.1), which was cloned, expressed, and leveraged as a coating protein for establishing an indirect enzyme-linked immunosorbent assay (ELISA) detection method. This method showcased outstanding specificity, repeatability, and sensitivity, exhibiting a notable agreement rate of 91.74% with the PRRSV IDEXX ELISA kit. The successful development of the NSP4 indirect ELISA not only supports the detection of PRRSV antibodies but also provides a robust platform for ongoing antibody monitoring in pig farming. Utilizing PRRSV NSP4 for ELISA antibody detection offers a more sustainable approach for continuous surveillance. The high agreement between this method and commercial kits lays a solid groundwork for effectively differentiating between inactivated and attenuated vaccines, enhancing the management and monitoring of PRRSV in pig populations.

Source: Frontiers in Microbiology

DOI: 10.3389/fmicb.2025.1549008

Authors: Mengmeng Zhao, Chen Lv, Jiankun Pang, Zhiyu Yang, Huiyang Sha

Institution: Foshan University

Language: English

Topic: Animal Virus Infections Studies

Subfield: Animal Science and Zoology

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,801