Work

DPV and EIS-based ultrasensitive aptasensor for VEGF165 detection based on nanoporous gold platform modified with SH-aptamer

Year: 2025

Type: article

Abstract: The sensitive detection of trace amounts of vascular endothelial growth factor (VEGF 165 ) in samples holds significant potential for clinical cancer diagnosis across various cancer types. This work introduces a novel and cost-effective dual strategy for developing an ultrasensitive electrochemical aptasensor based on the self-assembly of SH-aptamer onto a nanoporous gold electrode for VEGF 165 detection. SEM and electrochemical characterization confirmed the formation of the nanoporous structure and the increase of the surface area of the gold electrode from 0.1199 cm 2 to 0.1875 cm 2 . The sensing method relied on the interaction between the DNA probe and VEGF 165 , which increased the charge transfer resistance of Fe(CN) 6 3−/4- in the (signal-on) strategy, as determined by EIS. Conversely, it diminished the reduction peak current of the methylene blue probe in the (signal-off) strategy, as assessed by Differential Pulse Voltammetry (DPV). The limit of detection and linear dynamic range of the prepared electrode were found to be 0.25 pM and 2.5 to 140 pM, respectively, using DPV, and 0.18 pM and 5 to 200 pM, respectively, using EIS. The aptasensor demonstrated desirable stability after storing the aptasensor in a buffer solution for one week. Furthermore, analogous proteins did not exhibit significant interference. Also, detecting the VEGF 165 cancer marker in undiluted human serum samples had excellent recovery with relatively low standard deviation percentages. Therefore, the findings indicate that the proposed aptasensor could potentially be utilized in the future design of a non-invasive cancer diagnostic kit using a blood or interstitial fluid test instead of the painful and aggressive biopsy method.

Source: Sensing and Bio-Sensing Research

DOI: 10.1016/j.sbsr.2025.100766

Authors: Bahareh Pourbahram, Samira Mansouri Majd, Mojtaba Shamsipur

Institutions: Razi University, Pasargad Institute for Advanced Innovative Solutions

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,850