Year: 2025
Type: article
Abstract: Antibodies are valuable biological reagents used in a wide range of discovery research, biotechnology, diagnostic and therapeutic applications. Currently, both commercial and laboratory-scale antibody production is reliant on expression from mammalian cells, which can be time-consuming and requires the use of specialist facilities and costly growth reagents. Here, we describe a simple, rapid and cheap method for producing and isolating functional monoclonal antibodies and antibody fragments from bacterial cells that can be used in a range of laboratory applications. This simple method only requires access to basic microbial cell culture and molecular biology equipment, making scalable in-house antibody production accessible to the global diagnostics, therapeutics and molecular bioscience research communities.
Source: Open Biology
DOI: 10.1098/rsob.240229
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,839
Awards: Exceptional yield recombinant vesicle packaged protein export system for research and industrial biotechnology applications., G6443274824, Synthetic bacterial vesicles to enhance recombinant protein production, delivery and isolation for Industrial Biotechnology applications., MRC IAA 2021 University of Kent
OpenAlex ID: W4407747578