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WSSV Genotypes in Shrimp Aquaculture in Kerala, India Employing Variable Number Tandem Repeat in DNA Segments

Year: 2025

Type: article

Abstract: Disease caused by White spot syndrome virus (WSSV) is a major problem for the shrimp farming industry in India. Monitoring for the distribution and makeup of WSSV genotypes is thus important for identifying virus entry routes and developing strategies to avoid economic losses. Here variable number tandem repeat (VNTR) loci were analyzed to identify the variability and potential origins of WSSV genotypes occurring in shrimp farmed in Kerala. In total, 434 shrimp were screened by PCR. The study revealed that genetic characteristics of Taiwan, Indian and Chinese WSSV were prevalent in Kerala based on VNTR. Samples that are positive for WSSV in both first and second PCR itself were unable to produce amplifications for ORF94, 125 and75. This may be due to the sequence variation at the primer binding site. The potential use of VNTR loci analysis for the source tracking of WSSV in the various shrimp production system and recommends for bigger surveillance on genotyping to identify the unknown source of origin for better visualization of the distribution of shrimp.

Source: Journal of Advances in Biology & Biotechnology

DOI: 10.9734/jabb/2025/v28i22011

Authors: Akhilnath P.G., Shaheer. P., Reethy P. S., Murugadas V., Toms C. Joseph, K.V. Lalitha

Institutions: Central Institute of Fisheries Technology, Indian Council of Agricultural Research, Council of Scientific and Industrial Research, National Institute of Oceanography, Karpagam Academy of Higher Education

Language: English

Topic: Aquaculture disease management and microbiota

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

OpenAlex ID: W4407796834

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