Work

Quantitation of antibiotics in fresh fermentation medium by hydrophilic interaction chromatography mass spectrometry

Year: 2025

Type: article

Abstract: This study presents the development of a sophisticated liquid chromatography-mass spectrometry approach leveraging hydrophilic interaction chromatography (HILIC) for the quantification of kanamycin and spectinomycin in fermentation media. The method was validated per International Council for Harmonisation guidelines, demonstrating robust linearity, precision, and accuracy. To mitigate pronounced matrix effects common to complex fermentation matrices, sample preparation was thoroughly optimized with solid-phase extraction employing MCX sorbent, thereby enhancing recovery rates and minimizing analytical interference. The validated protocol demonstrated high correlation coefficients (R > 0.998), underscoring its robustness and reliability for the accurate quantification of antibiotics in challenging bioprocess environments, providing a valuable analytical tool for bioreactor system monitoring.

Source: Analytical and Bioanalytical Chemistry

DOI: 10.1007/s00216-025-05775-6

Authors: Nadia Marcon, Mathias Rüdt, Joachim Klein, Saša M. Miladinović

Institutions: HES-SO Valais-Wallis, Institute of Life Sciences, Lonza (Switzerland)

Language: English

Topic: Analytical Methods in Pharmaceuticals

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,990

Funder: Haute école Spécialisée de Suisse Occidentale