Italian White Truffle (Tuber magnatum Pico): Discovery of new molecules through untargeted UHPLC-QTOF-MS analysis
Year: 2025
Type: article
Abstract: The Italian white truffle ( Tuber magnatum Pico) is worldwide recognized as one of the most expensive edible mushrooms. Its chemical characterization has not yet been properly investigated in scientific literature. The aim of this study was to explore, for the first time, the truffle polar metabolic profile using an untargeted approach through UHPLC-ESI-QTOF-MS data-dependent acquisition. The total features list was filtered and molecules were annotated according to the MS spectra correspondence score with a threshold of 60 %. This led to a total of 412 annotated compounds mainly referred to amino acids, B-vitamins, carbohydrates and fatty acids derivatives. A targeted analysis was applied to four annotated molecules never detected in truffle before, being riboflavin, azelaic, 2-isopropylmalic and 3-isopropylmalic acids. Some constituents of the extracts were semi-quantitatively analysed by 1 H NMR spectroscopy. Results place a first brick in white truffle characterization, paving the way towards authenticity biomarkers detection useful in frauds prevention. • Untargeted LC-MS data-dependent analysis first-time applied to white truffle • Sample preparation with a cold extraction using 80 % methanol aqueous solution • A total of 412 compounds were annotated in truffle extracts by LC-MS • Four first-time detected compounds confirmed and quantified in Italian white truffle
Source: Food Chemistry
Institution: Università di Camerino
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,930
OpenAlex ID: W4407853807