Work

Biosafety assessment of edible oil blended from flax, coconut, sunflower, and olive oils in healthy Sprague Dawley rats

Year: 2025

Type: article

Abstract: Edible oil blending to formulate a health-protective fatty acid combination has opened new doors in food science. In this study, a 42-day biosafety assessment of a blended oil (BO) made from oils of coconut (CO), flaxseed (FO), olive (OO), and sunflower (SFO) was performed on healthy rats. Rats were given these oils as 100% fat replacement in control. The results of BO in rats encouraged its health protection properties by significantly lowering body weight gain (68.8±10.84), BMI (0.128±0.01), body fat content (-0.41±3.41), total cholesterol (43.00±7.84), triglycerides (90.00±9.00), total lipid contents (176.00±24.39), and LDL (14.00±4.12) while substantially improving HDL, LFTs, RFTs, and hematology when compared to single oils. BO consumption also indicated maximum protection against cardiovascular risk indices, including atherogenic (0.99±0.16) and coronary (3.07±0.21) indexes. Results for ECG also showed a normal cardiac rhythm in cardiac waves. Its regular consumption could help reduce the onset of obesity and metabolic diseases.

Source: Journal of Agriculture and Food Research

DOI: 10.1016/j.jafr.2025.101768

Authors: Roshina Rabail, Abdur Rauf Khalid, Amin Mousavi Khaneghah, Muhammad Faisal Manzoor, Gholamreza Abdi, Rana Muhammad Aadil