17 Self-fixing scaffold membrane prevents postoperative adhesion by scavenging ROS production
Year: 2025
Type: article
Abstract: Aim This study focuses on the investigation of formation mechanism and prevention technologies for postoperative abdominal adhesions by combining bioengineering technology with the target therapy on abdominal adhesion mechanism. Methods A tissue engineering anti-adhesion membrane (SC-Xg) was prepared by cross-linking xanthan gum (Xg) with sodium citrate (SC) through dehydration condensation reaction for in vivo implantation to prevent postoperative abdominal adhesions. The physicochemical properties of SC-Xg were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, rheometry, and swelling experiments to screen for suitable material formula ratios. Moreover, the biocompatibility of SC-Xg was assessed by co-culture with cells and fluorescence staining of live/dead cells. Through cellular experiments, we explored the possible mechanisms of sodium citrate inhibition of oxidative stress. Based on in vivo implantation of SC-XG membrane to mouse IBM, the safety and efficacy of SC-Xg in preventing postoperative abdominal adhesions were further confirmed using histopathological staining and ELISA assays. Results We developed a sodium citrate-based cross-linked xanthan gum tissue engineering membrane (SC-Xg) through a dehydration condensation reaction for the prevention of abdominal adhesion formation after abdominal surgery. The mechanical properties of xanthan gum were optimized by using different amount of sodium citrate for cross-linking. The SC-XG membrane showed the antioxidant properties, good self-fixation ability, biocompatibility, and biodegradability, and was easy to prepare for applications. In vitro and in vivo experiments confirmed the clinical translational potential of SC-Xg by shielding the surgical site, inhibiting oxidative stress and inflammatory response by activating Nrf2, and significantly reducing adhesion formation. Conclusions SC-Xg membrance inhibits oxidative stress in the injured peritoneum and prevents the formation of adhesions.
Source: British journal of surgery
Authors: Sicheng Li, Ye Liu, Jinjian Huang, Xiuwen Wu, Jianan Ren
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W4407902033