[Lycium barbarum Polysaccharide Improves Reproductive Injury in Female Rats Exposed to High-Altitude Hypoxic Environment: Investigation of the Mechanisms Involved].
Year: 2024
Type: other
Abstract: Objective: polysaccharide (LBP) on reproductive system damage induced by exposure to high-altitude hypoxic environment in female rats, and to explore the mechanisms involved. Methods: After undergoing physiological synchronization, 30 female Wistar rats were randomly and evenly assigned to 3 groups, including a plain control (C) group, a high-altitude hypoxia (H) group, and a high-altitude hypoxia + LBP (H-LBP) group. The C group was placed in a region at an altitude of 1500 m above sea level (with an oxygen volume fraction of 18.55%), while the H group and the H-LBP group were placed in a region at an altitude of 4010 m above sea level (with an oxygen volume fraction of 12.70%). Rats in the H-LBP group were fed with LBP at 75 mg/kg via gastric gavage, while the C and H groups received normal saline once a day for 14 days in a row. Changes in estrous cycles were documented throughout the experiment. At the end of the experiment, the serum levels of reproductive hormones and the levels of oxidative stress in the ovarian and uterine tissues were measured. Morphological changes in the ovarian and uterine tissues were assessed using hematoxylin-eosin (HE) staining. A component-target-pathway network diagram was constructed using network pharmacology methods to analyze the key targets and pathways. Results: <0.05), with LBP significantly improving the pathological damage to the reproductive organs of female rats caused by high-altitude hypoxic environment. Through network pharmacology analysis, we identified 76 potential targets for the protective effect of LBP against high-altitude hypoxia-induced reproductive injury, and the targets were mainly involved in the signaling pathways such as calcium channels, PI3K-Akt, MAPK, and HIF-1. Conclusion: LBP can ameliorate high-altitude hypoxia-induced reproductive damage in female rats. The mechanisms involved may be associated with the regulation of PI3K-Akt, MAPK, and HIF-1 pathways.
Source: PubMed
DOI: 10.12182/20241160203
Authors: Xiaojing Zhang, Yan Zhong, Hongfang Mu, Feifei Liu, Xiwen Chang, Rong Wang
Language: English
Topic: High Altitude and Hypoxia
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
Funder: Chinese People’s Liberation Army
OpenAlex ID: W4407908041