Flow Cytometry for Assessing Human Sperm Acrosomal Reaction (AR) and Correlation of AR and Acrosomal Enzyme Activity With Semen Parameters
Year: 2025
Type: article
Study design: Observational Study
Abstract: BACKGROUND/AIM: This study aimed to evaluate human sperm acrosomal reaction (AR) using flow cytometry and examine the correlation of AR and acrosomal enzyme activity (AEA) with seven semen parameters: total sperm count, concentration, vitality, motility, morphology, survival rate, and sperm DNA fragmentation index (DFI). PATIENTS AND METHODS: A retrospective analysis was conducted on semen data from 398 men in the AR group and 526 men in the AEA group. AR was assessed using flow cytometry, while AEA was measured with the solid-phase BAPNA method. Correlations between AR, AEA, and the seven semen parameters were analyzed. Subgroups based on standard reference values were also compared. RESULTS: >0.05). CONCLUSION: AEA shows stronger and more consistent correlations with semen parameters compared with AR, suggesting its potential as a reliable marker for evaluating male fertility. Further research is needed to clarify the predictive value of AR in male fertility assessment.
Source: In Vivo
Authors: ZUBIN HE, JINLIANG DUAN, XIN ZHOU, QINGDI QUENTIN LI, ZHUGUANG LIANG, HONGLIN CHEN, JIAN XIE, JIAQIANG LUO, YAOBIN WANG, GUANGZHI LIU, MEIFANG ZENG
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4407967149