Work

Biosynthesis of the C‐Glycosylated Depside Arenicolin B

Year: 2025

Type: article

Abstract: We uncovered the biosynthetic pathway of the C-glycosylated depside arenicolin B from Phialomyces arenicola through genome mining, heterologous reconstitution and biochemical characterization. The biosynthetic gene cluster (BGC) of arenicolin B is comprised of a HRPKS, a NRPKS, and a C-glycosyltransferase. Through biochemical dissection and evaluation, we demonstrate the NRPKS thioesterase (TE) domain catalyses depside formation.

Source: ChemBioChem

DOI: 10.1002/cbic.202500003

Authors: Colin W. Johnson, Lorraine Nuniz, Bruno Perlatti, Gerald F. Bills, Yi Tang

Institutions: University of California, Los Angeles, Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, Rutgers, The State University of New Jersey

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: G4305609567