Efficacy Evaluation of “Enhanced” Natural Killers with CISH and B2M Knockouts on Viability and Metabolic Status of 3D Glioblastoma Spheroid Cells in Patients
Year: 2025
Type: article
Abstract: was to evaluate the impact efficacy of "enhanced" NK cells on early metabolic rearrangements and the viability of glioblastoma cells in a patient using a tumor spheroid model. Materials and Methods: ) knockouts. Tumor spheroids were produced in round-bottomed, low-adhesive plates. 100 thousand immune cells were added to each spheroid, and spheroids viability was evaluated at several time points applying fluorescence staining using a live/dead cell viability assay kit; autofluorescence of metabolic coenzyme nicotinamide adenine dinucleotide (phosphate), or NAD(P)H, was visualized in spheroids using an LSM 880 laser scanning microscope (Carl Zeiss, Germany) with a FLIM module (Becker & Hickl GmbH, Germany). Results: , indicating occurrence of an early metabolic shift in tumor cells towards a less aggressive oxidative phenotype, and this is consistent with dead cells fraction increase and living cells fraction decrease in spheroid composition. Conclusion: The data obtained on enhanced cytotoxic activity of new modified NK cell lines against human glioblastoma spheroids are important to understand interaction mechanisms between tumor and immune cells and the development of glioblastoma adoptive cell therapy.
Institutions: Privolzhsky Research Medical University, Federal Medical-Biological Agency, N. I. Lobachevsky State University of Nizhny Novgorod, Peoples' Friendship University of Russia, Pirogov Russian National Research Medical University, Russian Academy of Sciences, Engelhardt Institute of Molecular Biology
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: diamond
Funder: Russian Science Foundation
OpenAlex ID: W4408032336