Work

Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model

Year: 2025

Type: article

Abstract: The chick embryo chorioallantoic membrane (CAM) is a viable alternative in vivo model to explore cancer biology due to its simplicity, accessibility, and cost-effectiveness. Here, we present a protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo CAM model. We describe steps for initial general preparation and for the angiogenic and tumorigenic assays in two distinct sections. • Steps for egg preparation for angiogenic assays • Instruction for tumor cell engraftment on the CAM • Steps for analyzing angiogenic and tumorigenic outcomes Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The chick embryo chorioallantoic membrane (CAM) is a viable alternative in vivo model to explore cancer biology due to its simplicity, accessibility, and cost-effectiveness. Here, we present a protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo CAM model. We describe steps for initial general preparation and for the angiogenic and tumorigenic assays in two distinct sections.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103663

Authors: Tommy Chastel, Serena Filiberti, Stefania Mitola, Roberto Ronca, Andrei Turtoi, Michela Corsini

Institutions: Inserm, Institut de Recherche en Cancérologie de Montpellier, University of Brescia

Language: English

Topic: Angiogenesis and VEGF in Cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550