Work

Luminos: open-source software for bidirectional microscopy

Year: 2025

Type: preprint

Abstract: Bidirectional microscopy (BDM) combines simultaneous targeted optical perturbation and imaging of biophysical or biochemical signals (e.g. membrane voltage, Ca 2+ , or signaling molecules). A core challenge in BDM is precise spatial and temporal alignment of stimulation, imaging, and other experimental parameters. Here we present Luminos, an open-source MATLAB library for modular and precisely synchronized control of BDM experiments. The system supports hardware-triggered synchronization across stimulation, recording, and imaging channels with microsecond accuracy. Source code and documentation for Luminos are available online at https://www.luminosmicroscopy.com and https://github.com/adamcohenlab/luminos-microscopy . This library will facilitate development of bidirectional microscopy methods across the biological sciences.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.1101/2025.02.22.639658

Authors: Daniel G. Itkis, F. Phil Brooks, Hunter C. Davis, Raphael Hotter, J. David Wong-Campos, Yitong Qi, Bill Z. Jia, Madeleine Howell, Marley Xiong, Rebecca Frank Hayward, Byung Hun Lee, Yangdong Wang, Rebecca T. Perelman, Adam E. Cohen

Institution: Harvard University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Neurological Disorders and Stroke

Awards: Chemigenetic voltage indicators for far-red and two-photon imaging in vivo, Two-Photon All-Optical Electrophysiology in Behaving Mice, Brain-wide correlation of single-cell firing properties to patterns of gene expression

OpenAlex ID: W4408042970

Cites: 31

Cited by: 4

Related to: 10