Work

Transposon mutagenesis identifies acid resistance and biofilm genes as Shigella sonnei virulence factors in Caenorhabditis elegans infection

Year: 2025

Type: article

Abstract: Identifying essential genes in bacterial pathogens during infection can enhance knowledge and provide novel targets for antimicrobial agents’ development. Currently, only Shigella flexneri essential genes during in vitro growth have been experimentally identified . This study used transposon insertion sequencing (TIS) to identify Shigella sonnei essential genes during Caenorhabditis elegans infection. 498 genes were predicted to be essential in S. sonnei during growth on nutrient-rich media. Some genes previously predicted to be essential in Shigella were found non-essential in S. sonnei, such as acetyl metabolism genes ( aceEF, lpdA ) and sulphate transport genes ( cysA, cyst, cysW ). Finally, 217 genes were predicted as S. sonnei virulence genes during infection, including acid resistance and biofilm formation genes which was not linked to S. sonnei virulence previously. • Among 498 S. sonnei essential genes, 121 are essential in S. flexneri and E. coli. • Some essential genes are specific in S. sonnei, demonstrating species differences. • Acid resistance and biofilm formation genes are involved in S. sonnei virulence. • These genes are not linked directly to S. sonnei pathogenesis previously.

Source: Biochemical and Biophysical Research Communications

DOI: 10.1016/j.bbrc.2025.151546

Authors: Bao Chi Wong, Fong Yoke Ling, Qasim Ayub, Hock Siew Tan

Institution: Monash University Malaysia

Language: English

Topic: Escherichia coli research studies

Subfield: Endocrinology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,110