Work

Critical Evaluation of Combined Cu and Fe3O4 Nanodots with Gum Arabic Nano-Hybrids Using in-vitro Model: Flow Cytometry and Cell Viability Studies

Year: 2025

Type: article

Abstract: Background: Previous studies on synthesis and cytotoxicity effect of copper (Cu) and magnetite (Fe 3 O 4 ) nanodots against kidney and lung cancer cells Lines. Purpose: Investigation of the effects of Cu and Fe 3 O 4 nanodots on the reduction in viability of lung cancer (A549) and human embryonic kidney (HEK239T) cells lines. Methods: Cu and Fe 3 O 4 nanodots/Gum Arabic (GA) hybrids were chemically synthesized, characterized, and assessed by MTT and flow cytometry for their cytotoxicity against A549 and HEK239T cells lines. Results: Novel hybrids of Cu and Fe 3 O 4 nanodots were effectively synthesized by chemical deposition method in combination with GA stabilizing agent. The stabilized Cu and Fe 3 O 4 nanodots by GA have median particle size of 4.7 nm and 7 nm respectively. Furthermore, the biological activities of Cu nanodots/ GA hybrids showed a strong reduction in viability for both A549 and HEK239T cells at 50 and 100 ng/μL, owing to their small size and high surface-area-to-volume ratio compared to Fe 3 O 4 nanoparticles. Pre-apoptosis effect of the Cu nanodots/ GA hybrid on the treated A549 cells were 40% for 0.1 ng/μL, 91.4% for 1 ng/μL, 88% for 50 ng/ μL, and 87.7% for 100 ng/μL. However, in the treated human embryonic kidney (HEK293T) cells, the pre-apoptosis was found under different concentrations conditions of 89.9% for 0.1 ng/μL, 94.2% for 1 ng/μL, 76.2% for 50 ng/μL and 70.5% for 100 ng/μL. After treatment of both A549 and HEK293T cells with 100 ng/μL of Cu nanodots/GA hybrid, many cell deaths and reformed nanoparticles crystals were observed. Conclusion: Cu nanodots/GA hybrid strongly reduce the viability of both A549 and HEK239T cells at 50 and 100 ng/μL. However, Fe 3 O 4 nanodots/GA hybrid did not show any significant effect. Keywords: Cu nanodots, Fe 3 O 4 nanodots, Arabic gum, lung cancer, human embryonic kidney cells

Source: International Journal of Nanomedicine

DOI: 10.2147/ijn.s498894