Work

Genetically-encoded markers for confocal visualization of single dense core vesicles

Year: 2025

Type: article

Abstract: Neuronal dense core vesicles (DCVs) store and release a diverse array of neuromodulators, trophic factors and bioamines. The analysis of single DCVs has largely been possible only using electron microscopy, which makes understanding cargo segregation and DCV heterogeneity difficult. To address these limitations, we developed genetically-encoded markers for DCVs that can be used in combination with standard immunohistochemistry and expansion microscopy, to enable single-vesicle resolution with confocal microscopy.

Source: Communications Biology

DOI: 10.1038/s42003-025-07829-y

Authors: Junwei Yu, Yunpeng Zhang, Kelsey Clements, Nannan Chen, Leslie C. Griffith

Institutions: Brandeis University, Southeast University

Language: English

Topic: Cellular transport and secretion

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $4,190

Funders: U.S. Department of Health and Human Services, National Institutes of Health, National Institute of Neurological Disorders and Stroke