Regional analysis of myelin basic protein across postnatal brain development of C57BL/6J mice
Year: 2025
Type: article
Abstract: Healthy brain development hinges on proper myelination, with disruption contributing to a wide array of neurological disorders. Immunohistochemical analysis of myelin basic protein (MBP) is a fundamental technique for investigating myelination and related disorders. However, despite decades of MBP research, detailed accounts of normal MBP progression in the developing mouse brain have been lacking. This study aims to address this gap by providing a detailed spatiotemporal account of MBP distribution across 13 developmental ages from postnatal day 2 to 60. We used an optimized immunohistochemistry protocol to overcome the challenges of myelin's unique lipid-rich composition, enabling more consistent staining across diverse brain structures and developmental stages, offering a robust baseline for typical myelination patterns, and enabling comparisons with pathological models. To support and potentially accelerate research into myelination disorders, we have made >1,400 high-resolution micrographs accessible online under the Creative Commons license.
Source: Frontiers in Neuroanatomy
Institution: University of North Carolina at Chapel Hill
Language: English
Subfield: Developmental Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
Funders: Simons Foundation, National Institutes of Health, National Institute of Mental Health, National Institute of Neurological Disorders and Stroke, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Eunice Kennedy Shriver National Institute of Child Health and Human Development
OpenAlex ID: W4408206697