Work

Dynamic exchange strategy for enzyme immobilization in Zr-based metal-organic frameworks for green synthesis of β-lactam antibiotics

Year: 2025

Type: article

Abstract: Penicillin G acylase (PGA), a crucial biocatalyst in β-lactam antibiotic synthesis, has been significantly limited in its industrial application due to inherent stability issues. To address this critical challenge, efficient immobilization strategies and suitable carriers should be thoroughly investigated and developed. In this study, a novel dynamic exchange strategy was successfully implemented to immobilize PGA onto Zr-based metal-organic frameworks (Zr-MOFs), resulting in the fabrication of a series of PGA-enzyme composites (PGA@Zr-MOFs). These composites exhibited remarkable stability while retaining 61%-85% of the free enzyme activity. The PGA@Zr-MOFs demonstrated exceptional catalytic performance in fixed-bed reactors under continuous operation conditions, maintaining 99% of their initial activity after 50 consecutive cycles. Furthermore, a cost-effective solid-phase mechanical ball milling approach was developed for the synthesis of Zr-MOFs, which achieved comparable performance to previously mentioned materials. The versatility of this immobilization strategy was further demonstrated with oxidoreductases (formate dehydrogenase (FDH) and alcohol dehydrogenase (ADH)), which are essential enzymes in β-lactam antibiotic production. The immobilized oxidoreductases retained 51%-55% of their free enzyme activity and maintained 97% of their activity after 50 continuous cycles in fixed-bed operations. This strategy offers a promising platform for the eco-friendly and sustainable industrial production of β-lactam antibiotics. • PGA@Zr-MOFs were successfully synthesized via dynamic exchange strategy, showing remarkable catalytic activity and stability. • Cost-effective protocols via aqueous-phase and ball milling synthesis enabled scalable production of biocatalysts. • Broad applicability of the proposed immobilization methodology has been demonstrated across various enzyme categories.

Source: Green Chemical Engineering

DOI: 10.1016/j.gce.2025.03.001

Authors: Heng Hu, Shaochun Wu, Yunlong Zheng, Zhenjie Zhang, Mingfang Yang, Yao Chen

Institutions: Chinese Academy of Sciences, Nankai University, State Key Laboratory of Medicinal Chemical Biology

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Responsible consumption and production

Open Access status: diamond

Funders: National Natural Science Foundation of China, China Postdoctoral Science Foundation, Nankai University, National Key Research and Development Program of China